|
KMID : 0545120150250081241
|
|
Journal of Microbiology and Biotechnology
2015 Volume.25 No. 8 p.1241 ~ p.1245
|
|
|
Identification of the σ70-Dependent Promoter Controlling Expression of the ansPAB Operon of the Nitrogen-Fixing Bacterium Rhizobium etli
|
|
Angelica Moreno-Enriquez
Zahaed Evangelista-Martinez
Luis Servin-Gonzalez
Maria Elena Flores-Carrasco
|
|
|
Abstract
|
|
|
|
The aim of the present work was to examine the putative promoter region of the operon ansPAB and to determine the general elements required for the regulation of transcriptional activity. The transcriptional start site of the ansPAB promoter was determined by using highresolution S1-nuclease mapping. Sequence analysis of this region showed -10 and -35 elements, which were consistent with consensus sequences for R. etli promoters that are recognized by the major form of RNA polymerase containing the σ70 transcription factor. Mutation studies affecting several regions located upstream of the transcriptional start site confirmed the importance of these elements on transcriptional expression.
|
|
|
KEYWORD
|
|
|
asparagine operon, σ70 transcription factor, Rhizobium etli, S1-nuclease mapping
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|